1 year post-doc position in Laboratoire Lésions des Acides Nucléiques (DSM/INAC/SCIB) CEA Grenoble.
Concern about nanoparticle (NPs) impact on human health leads to increasing demand of relevant toxicology data. In vitro studies published to date have been majorly processed on cells exposed to high concentrations of NPs, prepared as a liquid suspension. They show that NPs are cytotoxic, genotoxic and cause oxidative stress in exposed cells. We recently showed that TiO2-NPs also reduced cell capacity to repair damaged DNA.
The aim of this project is to validate these toxicological data on a more relevant model. Up to now we have worked on A549 lung alveolar cells, exposed to a suspension of TiO2-NPs. In the present project NP impact and particularly NP genotoxicity will be evaluated on A549 cells exposed to an aerosol of TiO2-NPs, at the air-liquid interface. NP deposition on cells will be carefully controlled and reduced to a dose which is relevant of occupational or population exposure. Cytotoxicity, NP intracellular accumulation and DNA damage induced by NPs will be evaluated on exposed cells, together with NP impact on DNA repair systems.
The techniques used for this project are classical cytotoxicity and genotoxicity assays (MTT, LDH, trypan blue, comet assay, micronucleus assay, oxidized bases measurement…), transmission electron microscopy (TEM), qPCR. This project will be developed in close collaboration with Karlsruhe Institute of Technology (KIT, Germany) and Laboratoire Francis Perrin (LFP, CEA Saclay).
The applicant holds a Ph.D. in cell biology, and experience in toxicology testing. Previous handling of nanoparticles would be a plus. Fluent English or German is required since some experiments will be performed with a German team.
The candidate must demonstrate a strong motivation, innovation capacity and autonomy.
Please send a curriculum and motivation letter to Dr Marie Carriere. Email: marie.carriere@cea.fr
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